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Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
Available in a variety of stationary phases that function in normal and/or reverse phase. MilliporeSigma™ Hibar™ HPLC Columns, 250-10 come with customized packing (please specify packing material) and are 250mmL x 10mmI.D.
Available in a variety of stationary phases that function in normal and/or reverse phase. MilliporeSigma™ Hibar™ HPLC Columns, 250-4.6 come with customized packing (please specify packing material) and are 250mmL x 4.6mmI.D.
Due to a mixed retention mechanism, selectivity differences for polar groups are more pronounced than on C8 and C18 columns. C1 columns require 20-30% less organic modifier to provide retention similar to C18 columns.
SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.
Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates.